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FACS analysis of HeLa cells using GTX83303 JAK3 antibody [5H2]. Blue : JAK3 Red : negative control
FACS analysis of HeLa cells using GTX83303 JAK3 antibody [5H2]. Blue : JAK3 Red : negative control
FACS analysis of HeLa cells using GTX83303 JAK3 antibody [5H2]. Blue : JAK3 Red : negative control

JAK3 antibody [5H2]

GTX83303
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
TargetJAK3
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Overview

  • Supplier
    GeneTex
  • Product Name
    JAK3 antibody [5H2]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1/500 - 1/2000. ICC/IF: 1/200 - 1/1000. FACS: 1/200 - 1/400. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5H2
  • Conjugate
    Unconjugated
  • Gene ID3718
  • Target name
    JAK3
  • Target description
    Janus kinase 3
  • Target synonyms
    JAK-3; JAK3_HUMAN; JAKL; Janus kinase 3 (a protein tyrosine kinase, leukocyte); leukocyte Janus kinase; LJAK; L-JAK; tyrosine-protein kinase JAK3
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP52333
  • Protein Name
    Tyrosine-protein kinase JAK3
  • Scientific Description
    The protein encoded by this gene is a member of the Janus kinase (JAK) family of tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction. It is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. Mutations in this gene are associated with autosomal SCID (severe combined immunodeficiency disease). [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of JAK3 using anti-JAK3 antibody (A02598-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates Lane 2: human A549 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JAK3 antigen affinity purified polyclonal antibody (Catalog # A02598-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JAK3 at approximately 125KD. The expected band size for JAK3 is at 125KD.
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