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ProductsBack/JAK3 Mouse Monoclonal Antibody [Clone ID: 5H2]
Western blot analysis using JAK3 mouse mAb against Jurkat cell lysate (1).
Western blot analysis using JAK3 mouse mAb against Jurkat cell lysate (1).
Western blot analysis using JAK3 mouse mAb against Jurkat cell lysate (1).

JAK3 Mouse Monoclonal Antibody [Clone ID: 5H2]

AM06376SU-N
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetJAK3
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    JAK3 Mouse Monoclonal Antibody [Clone ID: 5H2]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5H2
  • Gene ID3718
  • Target name
    JAK3
  • Target description
    Janus kinase 3
  • Target synonyms
    JAK-3, JAK3_HUMAN, JAKL, L-JAK, LJAK, tyrosine-protein kinase JAK3, Janus kinase 3 (a protein tyrosine kinase, leukocyte), leukocyte Janus kinase
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP52333
  • Protein Name
    Tyrosine-protein kinase JAK3
  • Scientific Description
    JAK3 mouse monoclonal antibody, clone 5H2, Ascites
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of JAK3 using anti-JAK3 antibody (A02598-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates Lane 2: human A549 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JAK3 antigen affinity purified polyclonal antibody (Catalog # A02598-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JAK3 at approximately 125KD. The expected band size for JAK3 is at 125KD.
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