More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/JNK1 + JNK3 Polyclonal Antibody
Paraformaldehyde-fixed, paraffin embedded human lung carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with \tJNK1 + 3 Polyclonal Antibody, Unconjugated (bs-0501R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human lung carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with \tJNK1 + 3 Polyclonal Antibody, Unconjugated (bs-0501R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human lung carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with \tJNK1 + 3 Polyclonal Antibody, Unconjugated (bs-0501R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.

JNK1 + JNK3 Polyclonal Antibody

BS-0501R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetMAPK8
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    JNK1 + 3 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID5599
  • Target name
    MAPK8
  • Target description
    mitogen-activated protein kinase 8
  • Target synonyms
    c-Jun N-terminal kinase 1; JNK; JNK1; JNK1A2; JNK21B1/2; JNK-46; JUN N-terminal kinase; MAP kinase 8; mitogen-activated protein kinase 8; PRKM8; SAPK1; SAPK1c; stress-activated protein kinase 1; stress-activated protein kinase 1c
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP45983
  • Protein Name
    Mitogen-activated protein kinase 8
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Rhein ameliorates lipopolysaccharide-induced intestinal barrier injury via modulation of Nrf2 and MAPKs. Zhuang S et al., 2019 Jan 1, Life Sci
    Read more

Related products

Product group Antibodies
Biomatik
MAPK8 Polyclonal AntibodyCAC14610
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetMAPK8
  • SizePrice
Product group Antibodies
Assay Genie
JNK1 Polyclonal AntibodyCAB0288
TargetMAPK8
  • SizePrice
Product group Antibodies
MedChem Express
JNK1+JNK2+JNK3 AntibodyHY-P80197
TargetMAPK8
  • SizePrice
Western Blot analysis of hela cells using JNK1/2/3 Polyclonal Antibody
Product group Antibodies
Cusabio
MAPK8/MAPK9/MAPK10 AntibodyCSB-PA003084
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetMAPK8
  • SizePrice
Product group Antibodies
References
GeneTex
JNK1 + JNK2 antibodyGTX133806
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetMAPK8
  • SizePrice
Product group Antibodies
Biosynth
JNK1 antibody20-JS50
  • SizePrice
Product group Antibodies
RayBiotech
Anti-MAPK8 Antibody144-00288
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
TargetMAPK8
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
MAPK8 / JNK1 Antibody (Preservative Free)LS-C343354
ApplicationsELISA
TargetMAPK8
  • SizePrice
Figure 1. Western blot analysis of JNK1/2/3 using anti-JNK1/2/3 antibody (M02608-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U87 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse Neuro-2a whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JNK1/2/3 antigen affinity purified monoclonal antibody (Catalog # M02608-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JNK1/2/3 at approximately 40, 48 kDa. The expected band size for JNK1/2/3 is at 48 kDa.
Product group Antibodies
References
Boster Bio
Anti-JNK1/2/3 MAPK8 Rabbit Monoclonal AntibodyM02608-1
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
TargetMAPK8
  • SizePrice
Anti-JNK1 Antibody
Product group Antibodies
Antibodies.com
Anti-JNK1 AntibodyA29715
ApplicationsWestern Blot
  • SizePrice