jRCaMP1b-P2A-LOCa3 (coexpressing LOCa3 and a state-of-the-art red calcium indicator jRCaMP1b)

Applied Biological Materials

jRCaMP1b-P2A-LOCa3 (coexpressing LOCa3 and a state-of-the-art red calcium indicator jRCaMP1b)

9

Research Use Only

This Lentiviral vector is for co-expressing LOCa3 and a green genetically-encoded calcium sensor jRCaMP7c using a P2A self-cleaving bicistronic expression cassette LOCa3 is a LOV2-ORAI1( H171D/P245T) hybrid construct that functions as a light-operated Ca2+ channel. This construct was cloned by inserting the light-oxygen-voltage domain (LOV2) of Avena sativa phototropin 1 into the intracellular loop of an engineered ORAI1 channel at residues S163 and P164. LOCa3 displays biophysical features reminiscent of the ORAI1 channel, which enables precise optical control over Ca2+ signals and hallmark Ca2+-dependent physiological responses. This vector was submitted to the abmXchange program by Yubin Zhou, Center for Translational Cancer Research, Institute of Biosciences and Technology, Texas A&M University, Houston, TX, USA

-20°C

41106600