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ICC/IF analysis of methanol-fixed HeLa cells using GTX50105 JunB (phospho Ser79) antibody.
ICC/IF analysis of methanol-fixed HeLa cells using GTX50105 JunB (phospho Ser79) antibody.
ICC/IF analysis of methanol-fixed HeLa cells using GTX50105 JunB (phospho Ser79) antibody.

JunB (phospho Ser79) antibody

GTX50105
GeneTex
ApplicationsImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetJUNB
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Overview

  • Supplier
    GeneTex
  • Product Name
    JunB (phospho Ser79) antibody - Orthogonal Validated
  • Delivery Days Customer
    9
  • Application Supplier Note
    ICC/IF: 1:100-1:200. IHC-P: 1:50-1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3726
  • Target name
    JUNB
  • Target description
    JunB proto-oncogene, AP-1 transcription factor subunit
  • Target synonyms
    activator protein 1; AP-1; jun B proto-oncogene; transcription factor jun-B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP17275
  • Protein Name
    Transcription factor jun-B
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of JUNB using anti-JUNB antibody (A01825-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JUNB antigen affinity purified polyclonal antibody (Catalog # A01825-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JUNB at approximately 43-45KD. The expected band size for JUNB is at 36KD.
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