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ProductsBack/Kallikrein 8 antibody [6G15]

Kallikrein 8 antibody [6G15]

GTX52889
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetKLK8
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Overview

  • Supplier
    GeneTex
  • Product Name
    Kallikrein 8 antibody [6G15]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    6G15
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID11202
  • Target name
    KLK8
  • Target description
    kallikrein related peptidase 8
  • Target synonyms
    HNP; kallikrein-8; NP; NRPN; ovasin; PRSS19; serine protease 19; serine protease TADG-14; TADG14; tumor-associated differentially expressed gene 14 protein
  • Host
    Mouse
  • Isotype
    IgG2
  • Protein IDO60259
  • Protein Name
    Kallikrein-8
  • Scientific Description
    Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in tandem in a gene cluster on chromosome 19. The encoded protein may be involved in proteolytic cascade in the skin and may serve as a biomarker for ovarian cancer. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Kallikrein 8 using anti-Kallikrein 8 antibody (PB10005). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kallikrein 8 antigen affinity purified polyclonal antibody (Catalog # PB10005) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kallikrein 8 at approximately 28 kDa. The expected band size for Kallikrein 8 is at 28 kDa.
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