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KAT9/Elp3 Rabbit mAb

A0020
ABclonal
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetELP3
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Overview

  • Supplier
    ABclonal
  • Product Name
    KAT9/Elp3 Rabbit mAb
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID55140
  • Target name
    ELP3
  • Target description
    elongator acetyltransferase complex subunit 3
  • Target synonyms
    KAT9, elongator complex protein 3, elongation protein 3 homolog, protein lysine acetyltransferase ELP3, tRNA uridine(34) acetyltransferase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H9T3
  • Protein Name
    Elongator complex protein 3
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ELP3 using anti-ELP3 antibody (A02833-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human K562 whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: human U-87MG whole cell lysates, Lane 7: human Caco-2 whole cell lysates, Lane 8: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ELP3 antigen affinity purified polyclonal antibody (Catalog # A02833-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ELP3 at approximately 62KD. The expected band size for ELP3 is at 62KD.
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