KBM ADSC-2 (Serum-free Adipose-derived stem cell culture medium)

Cosmo Bio USA

KBM ADSC-2 (Serum-free Adipose-derived stem cell culture medium)

16

Research Use Only

Adipose-derived stem cell culture medium KBM ADSC-1&2 Long-term culture is possible while maintaining undifferentiated state Manufacturer: Kohjin Bio Co., Ltd. This product is a culture medium suitable for growing adipose-derived stem cells while maintaining their undifferentiation. The maintenance of undifferentiation is confirmed by the fact that cells cultured in this medium differentiate into adipocytes, osteoblasts and chondrocytes, and by their surface markers. KBM ADSC-1&2, a culture medium for adipose-derived stem cells, enables long-term culture while maintaining the undifferentiated state of adipose-derived stem cells (ADSC) (*) . KBM ADSC-1 is a ready-to-use medium containing 5% FBS. We also sell KBM ADSC-2, which is KBM ADSC-1 without FBS . (*) Differentiation into fat, bone, and cartilage confirmed Intended use It can be used for research on differentiation induction (fat, bone, cartilage, etc.) and lipid production. Culture examples Example of culture using KBM ADSC-1 Figure 1 Growth curve during subculture Figure 2. Changes in PDT (population doubling time) during subculture Culture conditions Cells: ADSC (commercially available P1 frozen cells) Container: 6-well cell culture plate (Corning, product number: 3516) Culture: 4.75 x 104 cells were seeded in a 6-well cell culture plate. On day 3 of culture, the cells were counted, subcultured, and analyzed for surface markers by flow cytometry. Figure 3 (a) P3 ADSCs on day 3 of culture (b) P5 cells on day 7 after induction of adipogenic differentiation (Oil Red O staining) (c) P5 cells on day 21 after induction of osteogenic differentiation (Alizarin Red S staining) (d) P3 cells on day 28 after induction of chondrogenic differentiation (Alcian Blue staining) Culture conditions Cells: ADSCs (commercially available P1 frozen cells) cultured for 2 passages in KBM ADSC-1. Container: 24-well cell culture plate (FALCON, product number: 353047) culture: 6.25 x 104 fat cells were seeded on a 24-well cell culture plate. On the first day of culture in culture medium (D-MEM(H) + 10% FBS + 1mM Na pyruvate), differentiation induction was started with KBM Adipogenic-DM (tentative), the medium was changed every 2-3 days, and on the 7th day of culture, staining was done with Oil Red O. 6.25 x 104 bone cells were seeded in a 24-well cell culture plate. On the first day of culture in culture medium (D-MEM(H) + 10% FBS), differentiation induction was started with KBM Osteogenic-DM1 (tentative), and the medium was changed every 2 to 3 days. On the 21st day of culture, the cells were stained with Alizarin Red S. 6.25 x 104 cells of cartilage were seeded onto a 24-well cell culture plate. On the first day of culture in culture medium (D-MEM(H) + 10% FBS), differentiation induction was started with a general cartilage differentiation induction medium, the medium was changed every 3 to 4 days, and on the 28th day of culture, staining was performed with Alcian Blue. Culture example using KBM ADSC-2 Figure 4 Growth curve during subculture Figure 5. Changes in PDT (population doubling time) during subculture Culture conditions Cells: ADSC (commercially available P2 frozen cells) Container: 6-well cell culture plate (Corning, product number: 3516) Culture: 4.75 x 104 cells were seeded in a 6-well cell culture plate. On the third day of culture, the cells were counted, subcultured, and analyzed for surface markers by flow cytometry. Figure 6 (a) ADSC-2 + ADSCs on day 3 of P3 culture cultured with 5% FBS (b) ADSC-2 + ADSCs on day 3 of P3 culture cultured with 5% HS (c) HS P8 cells on day 7 after induction of adipogenic differentiation (Oil Red O staining) (d) HS P8 cells on day 21 after induction of osteogenic differentiation (Alizarin Red S staining) Culture conditions Cells: ADSCs (commercially available P2 frozen cells) cultured for 1-5 passages in KBM ADSC-2 + 5% FBS or KBM ADSC-2 + 5% HS Container: 24-well cell culture plate (FALCON, product number: 353047) culture: 6.25 x 104 fat cells were seeded onto a 24-well cell culture plate. On the first day of culture in culture medium (D-MEM(H) + 10% FBS), differentiation induction was started with KBM Adipogenic-DM (tentative), and the medium was changed every 2-3 days. On the 7th day of culture, staining was performed with Oil Red O. 6.25 x 104 bone cells were seeded in a 24-well cell culture plate. On the first day of culture in culture medium (D-MEM(H) + 10% FBS), differentiation induction was started with KBM Osteogenic-DM (tentative), and the medium was changed every 2 to 3 days. On the 21st day of culture, the cells were stained with Alizarin Red S. Adipose-derived stem cell culture medium KBM ADSC-1 (5% FBM) Adipose-derived stem cell culture medium KBM ADSC-2 (FBS-free)

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