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Western blot All lanes: KBTBD2 antibody at 2microg/ml + A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 72 kDa Observed band size: 72 kDa
Western blot All lanes: KBTBD2 antibody at 2microg/ml + A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 72 kDa Observed band size: 72 kDa
Western blot All lanes: KBTBD2 antibody at 2microg/ml + A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 72 kDa Observed band size: 72 kDa

KBTBD2 Antibody

CSB-PA811624LA01HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetKBTBD2
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Overview

  • Supplier
    Cusabio
  • Product Name
    KBTBD2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID25948
  • Target name
    KBTBD2
  • Target description
    kelch repeat and BTB domain containing 2
  • Target synonyms
    BKLHD1, kelch repeat and BTB domain-containing protein 2, BTB and kelch domain containing 1, BTB and kelch domain-containing protein 1, kelch repeat and BTB (POZ) domain containing 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IY47
  • Protein Name
    Kelch repeat and BTB domain-containing protein 2
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of KBTBD2 using anti-KBTBD2 antibody (A15277-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human COLO320 whole cell lysates, Lane 5: human SW620 whole cell lysates, Lane 6: human Caco-2 whole cell lysates, Lane 7: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KBTBD2 antigen affinity purified polyclonal antibody (Catalog # A15277-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KBTBD2 at approximately 72 kDa. The expected band size for KBTBD2 is at 71 kDa.
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