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Western Blot analysis of 293 cells using Kv1.3 Polyclonal Antibody
Western Blot analysis of 293 cells using Kv1.3 Polyclonal Antibody
Western Blot analysis of 293 cells using Kv1.3 Polyclonal Antibody

KCNA3 Antibody

CSB-PA003118
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetKCNA3
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Overview

  • Supplier
    Cusabio
  • Product Name
    KCNA3 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3738
  • Target name
    KCNA3
  • Target description
    potassium voltage-gated channel subfamily A member 3
  • Target synonyms
    HGK5, HLK3, HPCN3, HUKIII, KV1.3, MK3, PCN3, potassium voltage-gated channel subfamily A member 3, RP11-284N8.3, potassium channel 3, potassium channel, voltage gated shaker related subfamily A, member 3, potassium voltage-gated channel, shaker-related subfamily, member 3, type n potassium channel, voltage-gated K(+) channel HuKIII, voltage-gated potassium channel protein Kv1.3, voltage-gated potassium channel subunit Kv1.3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP22001
  • Protein Name
    Potassium voltage-gated channel subfamily A member 3
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of KCNA3 using anti-KCNA3 antibody (PB9650). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Mouse Brain Tissue Lysate at 50ug, Lane 3: K562 Whole Cell Lysate at 40ug, Lane 4: HELA Whole Cell Lysate at 40ug, Lane 5: 22RV1 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNA3 antigen affinity purified polyclonal antibody (Catalog # PB9650) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCNA3 at approximately 55 kDa. The expected band size for KCNA3 is at 64 kDa.
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