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Western Blot analysis of 293 cells using KV1.5 Polyclonal Antibody
Western Blot analysis of 293 cells using KV1.5 Polyclonal Antibody
Western Blot analysis of 293 cells using KV1.5 Polyclonal Antibody

KCNA5 Antibody

CSB-PA003119
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetKCNA5
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Overview

  • Supplier
    Cusabio
  • Product Name
    KCNA5 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3741
  • Target name
    KCNA5
  • Target description
    potassium voltage-gated channel subfamily A member 5
  • Target synonyms
    ATFB7; cardiac potassium channel; HCK1; HK2; HPCN1; insulinoma and islet potassium channel; KV1.5; PCN1; potassium channel 1; potassium channel, voltage gated shaker related subfamily A, member 5; potassium voltage-gated channel subfamily A member 5; potassium voltage-gated channel, shaker-related subfamily, member 5; voltage-gated potassium channel HK2; voltage-gated potassium channel protein Kv1.5; voltage-gated potassium channel subunit Kv1.5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP22460
  • Protein Name
    Potassium voltage-gated channel subfamily A member 5
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KCNA5 using anti-KCNA5 antibody (PB9651). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: 293T Whole Cell Lysate at 40ug, Lane 2: A549 Whole Cell Lysate at 40ug, Lane 3: PANC Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNA5 antigen affinity purified polyclonal antibody (Catalog # PB9651) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCNA5 at approximately 67 kDa. The expected band size for KCNA5 is at 67 kDa.
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