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Western Blot analysis of 3T3 cells using KV4.1 Polyclonal Antibody
Western Blot analysis of 3T3 cells using KV4.1 Polyclonal Antibody
Western Blot analysis of 3T3 cells using KV4.1 Polyclonal Antibody

KCND1 Antibody

CSB-PA010503
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetKCND1
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Overview

  • Supplier
    Cusabio
  • Product Name
    KCND1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3750
  • Target name
    KCND1
  • Target description
    potassium voltage-gated channel subfamily D member 1
  • Target synonyms
    KV4.1, A-type voltage-gated potassium channel KCND1, Shal-type potassium channel, potassium channel, voltage gated Shal related subfamily D, member 1, potassium voltage-gated channel, Shal-related subfamily, member 1, shal-type potassium channel KCND1, voltage-gated potassium channel subunit Kv4.1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NSA2
  • Protein Name
    A-type voltage-gated potassium channel KCND1
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of KCND1 using anti-KCND1 antibody (A11599-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCND1 antigen affinity purified polyclonal antibody (Catalog # A11599-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCND1 at approximately 71 kDa. The expected band size for KCND1 is at 71 kDa.
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