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Western Blot analysis of HuvEc cells using KCNH1 Polyclonal Antibody
Western Blot analysis of HuvEc cells using KCNH1 Polyclonal Antibody
Western Blot analysis of HuvEc cells using KCNH1 Polyclonal Antibody

KCNH1 Antibody

CSB-PA003093
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetKCNH1
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Overview

  • Supplier
    Cusabio
  • Product Name
    KCNH1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3756
  • Target name
    KCNH1
  • Target description
    potassium voltage-gated channel subfamily H member 1
  • Target synonyms
    EAG, EAG1, K(V)10.1, Kv10.1, TMBTS, ZLS1, h-eag, hEAG, hEAG1, voltage-gated delayed rectifier potassium channel KCNH1, EAG channel 1, ether-a-go-go 1, ether-a-go-go potassium channel 1, ether-a-go-go, Drosophila, homolog of, potassium channel, voltage gated eag related subfamily H, member 1, potassium voltage-gated channel, subfamily H (eag-related), member 1, voltage-gated potassium channel subunit Kv10.1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95259
  • Protein Name
    Voltage-gated delayed rectifier potassium channel KCNH1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of KCNH1 using anti-KCNH1 antibody (A01036-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human COLO-320 cell lysate, Lane 2: human HepG2 cell lysate, Lane 3: human A549 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNH1 antigen affinity purified polyclonal antibody (Catalog # A01036-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCNH1 at approximately 150KD. The expected band size for KCNH1 is at 111KD.
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