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Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA889100DSR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA889100DSR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA889100DSR1HU at dilution of 1:100

KCNIP2 Antibody

CSB-PA889100DSR1HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetKCNIP2
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Overview

  • Supplier
    Cusabio
  • Product Name
    KCNIP2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID30819
  • Target name
    KCNIP2
  • Target description
    potassium voltage-gated channel interacting protein 2
  • Target synonyms
    A-type potassium channel modulatory protein 2; cardiac voltage-gated potassium channel modulatory subunit; KCHIP2; Kv channel interacting protein 2; Kv channel-interacting protein 2; potassium channel-interacting protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NS61
  • Protein Name
    Kv channel-interacting protein 2
  • Scientific Description
    Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels. Modulates channel density, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner. In vitro, modulates KCND2/Kv4.2 and KCND3/Kv4.3 currents. Involved in KCND2 and KCND3 trafficking to the cell surface. May be required for the expression of I(To) currents in the heart (By similarity).
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KCNA5 using anti-KCNA5 antibody (PB9652). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Rat Cardiac Muscle Tissue Lysate at 50ug, Lane 3: Mouse Cardiac Muscle Tissue Lysate at 50ug, Lane 4: 22RV1 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNA5 antigen affinity purified polyclonal antibody (Catalog # PB9652) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCNA5 at approximately 31 kDa. The expected band size for KCNA5 is at 31 kDa.
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