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Western blot All lanes: KCNJ1 antibody at 2microg/ml Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 45, 43 kDa Observed band size: 45 kDa
Western blot All lanes: KCNJ1 antibody at 2microg/ml Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 45, 43 kDa Observed band size: 45 kDa
Western blot All lanes: KCNJ1 antibody at 2microg/ml Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 45, 43 kDa Observed band size: 45 kDa

KCNJ1 Antibody

CSB-PA012047LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetKCNJ1
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Overview

  • Supplier
    Cusabio
  • Product Name
    KCNJ1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3758
  • Target name
    KCNJ1
  • Target description
    potassium inwardly rectifying channel subfamily J member 1
  • Target synonyms
    KIR1.1, ROMK, ROMK1, ATP-sensitive inward rectifier potassium channel 1, ATP-regulated potassium channel ROM-K, inward rectifier K(+) channel Kir1.1, inwardly rectifying K+ channel, potassium channel, inwardly rectifying subfamily J member 1, potassium voltage-gated channel subfamily J member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP48048
  • Protein Name
    ATP-sensitive inward rectifier potassium channel 1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of KCNJ1 using anti-KCNJ1 antibody (A02788-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: mouse kidney tissue lysates, Lane 4: monkey kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNJ1 antigen affinity purified polyclonal antibody (Catalog # A02788-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCNJ1 at approximately 45KD. The expected band size for KCNJ1 is at 45KD.
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