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ProductsBack/KDM4A Mouse Monoclonal Antibody [Clone ID: 5H1]
Western blot analysis using KDM4A antibody Cat.-No AM06496SU-N against HEK293 (1) and KDM4A (AA: 500-705)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot analysis using KDM4A antibody Cat.-No AM06496SU-N against HEK293 (1) and KDM4A (AA: 500-705)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot analysis using KDM4A antibody Cat.-No AM06496SU-N against HEK293 (1) and KDM4A (AA: 500-705)-hIgGFc transfected HEK293 (2) cell lysate.

KDM4A Mouse Monoclonal Antibody [Clone ID: 5H1]

AM06496SU-N
OriGene
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetKDM4A
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    KDM4A Mouse Monoclonal Antibody [Clone ID: 5H1]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5H1
  • Gene ID9682
  • Target name
    KDM4A
  • Target description
    lysine demethylase 4A
  • Target synonyms
    JHDM3A, JMJD2, JMJD2A, TDRD14A, lysine-specific demethylase 4A, [histone H3]-trimethyl-L-lysine(36) demethylase 4A, [histone H3]-trimethyl-L-lysine(9) demethylase 4A, jmjC domain-containing histone demethylation protein 3A, jumonji C domain-containing histone demethylase 3A, jumonji domain containing 2, jumonji domain containing 2A, jumonji domain-containing protein 2A, lysine (K)-specific demethylase 4A, tudor domain containing 14A
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDO75164
  • Protein Name
    Lysine-specific demethylase 4A
  • Scientific Description
    KDM4A mouse monoclonal antibody, clone 5H1, Ascites
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of JHDM3A/JMJD2A/KDM4A using anti-JHDM3A/JMJD2A/KDM4A antibody (A02850-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JHDM3A/JMJD2A/KDM4A antigen affinity purified polyclonal antibody (Catalog # A02850-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JHDM3A/JMJD2A/KDM4A at approximately 150 kDa. The expected band size for JHDM3A/JMJD2A/KDM4A is at 121 kDa.
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