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KDM6A / UTX Antibody (N-Terminus)

LS-C116898
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetKDM6A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    KDM6A / UTX Antibody (N-Terminus)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
  • Applications
    Western Blot
  • Applications Supplier
    WB (0.2 - 1 µg/ml) Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID7403
  • Target name
    KDM6A
  • Target description
    lysine demethylase 6A
  • Target synonyms
    [histone H3]-trimethyl-L-lysine(27) demethylase 6A; bA386N14.2; bA386N14.2 (ubiquitously transcribed X chromosome tetratricopeptide repeat protein (UTX)); histone demethylase UTX; KABUK2; lysine (K)-specific demethylase 6A; lysine-specific demethylase 6A; ubiquitously transcribed tetratricopeptide repeat protein X-linked; ubiquitously-transcribed TPR gene on the X chromosome; UTX
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of UTX/KDM6A using anti-UTX/KDM6A antibody (A01286-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HEK293 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UTX/KDM6A antigen affinity purified polyclonal antibody (Catalog # A01286-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UTX/KDM6A at approximately 154 kDa. The expected band size for UTX/KDM6A is at 154 kDa.
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