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ProductsBack/KDM6A antibody [C2C3], C-term
Sample (30 μg of whole cell lysate) A: IMR32 B: U87-MG 5% SDS PAGE GTX120873 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Sample (30 μg of whole cell lysate) A: IMR32 B: U87-MG 5% SDS PAGE GTX120873 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Sample (30 μg of whole cell lysate) A: IMR32 B: U87-MG 5% SDS PAGE GTX120873 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

KDM6A antibody [C2C3], C-term

GTX120873
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetKDM6A
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Overview

  • Supplier
    GeneTex
  • Product Name
    KDM6A antibody [C2C3], C-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7403
  • Target name
    KDM6A
  • Target description
    lysine demethylase 6A
  • Target synonyms
    KABUK2, UTX, bA386N14.2, lysine-specific demethylase 6A, [histone H3]-trimethyl-L-lysine(27) demethylase 6A, bA386N14.2 (ubiquitously transcribed X chromosome tetratricopeptide repeat protein (UTX)), histone demethylase UTX, lysine (K)-specific demethylase 6A, ubiquitously transcribed tetratricopeptide repeat protein X-linked, ubiquitously-transcribed TPR gene on the X chromosome
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15550
  • Protein Name
    Lysine-specific demethylase 6A
  • Scientific Description
    This gene is located on the X chromosome and is the corresponding locus to a Y-linked gene which encodes a tetratricopeptide repeat (TPR) protein. The encoded protein of this gene contains a JmjC-domain and catalyzes the demethylation of tri/dimethylated histone H3. [provided by RefSeq]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Shelar S, Shim EH, Brinkley GJ, et al. Biochemical and Epigenetic Insights into L-2-Hydroxyglutarate, a Potential Therapeutic Target in Renal Cancer. Clin Cancer Res. 2018,24(24):6433-6446. doi: 10.1158/1078-0432.CCR-18-1727
    Read this paper

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Figure 1. Western blot analysis of UTX/KDM6A using anti-UTX/KDM6A antibody (A01286-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HEK293 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UTX/KDM6A antigen affinity purified polyclonal antibody (Catalog # A01286-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UTX/KDM6A at approximately 154 kDa. The expected band size for UTX/KDM6A is at 154 kDa.
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