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Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Human normal stomach tissue, Primary antibody: CSB-PA064874(KDM8 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Human normal stomach tissue, Primary antibody: CSB-PA064874(KDM8 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Human normal stomach tissue, Primary antibody: CSB-PA064874(KDM8 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes

KDM8 Antibody

CSB-PA064874
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetKDM8
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Overview

  • Supplier
    Cusabio
  • Product Name
    KDM8 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID79831
  • Target name
    KDM8
  • Target description
    lysine demethylase 8
  • Target synonyms
    JMJD5, bifunctional peptidase and arginyl-hydroxylase JMJD5, JmjC arginyl-hydroxylase, L-arginine (3R)-hydroxylase KDM8, arginyl C3-hydroxylase KDM8, jmjC domain-containing protein 5, jumonji C domain-containing protein 5, jumonji domain containing 5, jumonji domain-containing protein 5, lysine (K)-specific demethylase 8, lysine-specific demethylase 8
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N371
  • Protein Name
    JmjC domain-containing protein 5
  • Scientific Description
    This gene likely encodes a histone lysine demethylase. Studies of a similar protein in mouse indicate a potential role for this protein as a tumor suppressor. Alternatively spliced transcript variants have been described.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of KDM8 using anti-KDM8 antibody (A08840-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KDM8 antigen affinity purified polyclonal antibody (Catalog # A08840-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KDM8 at approximately 55 kDa. The expected band size for KDM8 is at 47 kDa.
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