Immunofluorescence microscopy using GeneTex Immunochemicals Mouse Mab-anti-Keratin antibody. Confocal slices of HeLa cells are between 0.5 and 0.6 um where the image is taken near the bottom of the cell. Use FITC a 1:2,000 dilution of FITC-conjugated Goat anti-Mouse IgG [H&L] for detection.
![Western blot using GeneTex Immunochemicals Mouse Mab-anti-Keratin antibody. This antibody recognizes a single 56 kDa band corresponding to human keratin as confirmed by the position of molecular weight markers (not shown). Approximatley 100 microg of keratin from human epidermis was applied under reducing conditions. 1:400 dilution of Mab anti-Keratin was used for 2h followed by detection using a 1:5,000 dilution of IRDyeTM800 conjugated Goat anti-Mouse IgG [H&L] and visualization using the OdysseyR Infrared Imaging System developed by LI-COR.](https://www.genetex.com/upload/website/prouct_img/normal/GTX48825/GTX48825_20160330_WB_w_23060823_385.webp "Western blot using GeneTex Immunochemicals Mouse Mab-anti-Keratin antibody. This antibody recognizes a single 56 kDa band corresponding to human keratin as confirmed by the position of molecular weight markers (not shown). Approximatley 100 microg of keratin from human epidermis was applied under reducing conditions. 1:400 dilution of Mab anti-Keratin was used for 2h followed by detection using a 1:5,000 dilution of IRDyeTM800 conjugated Goat anti-Mouse IgG [H&L] and visualization using the OdysseyR Infrared Imaging System developed by LI-COR.")
![ICC/IF analysis of HeLa cells using GTX48825 pan Cytokeratin antibody [C-11].](https://www.genetex.com/upload/website/prouct_img/normal/GTX48825/GTX48825_20240423_ICCIF_323_24042320_116.webp "ICC/IF analysis of HeLa cells using GTX48825 pan Cytokeratin antibody [C-11].")
![WB analysis of recombinant human pan Cytokeratin protein using GTX48825 pan Cytokeratin antibody [C-11]. Loading : 100 ng Dilution : 1:400](https://www.genetex.com/upload/website/prouct_img/normal/GTX48825/GTX48825_20240423_WB_324_24042320_534.webp "WB analysis of recombinant human pan Cytokeratin protein using GTX48825 pan Cytokeratin antibody [C-11]. Loading : 100 ng Dilution : 1:400")
Immunofluorescence microscopy using GeneTex Immunochemicals Mouse Mab-anti-Keratin antibody. Confocal slices of HeLa cells are between 0.5 and 0.6 um where the image is taken near the bottom of the cell. Use FITC a 1:2,000 dilution of FITC-conjugated Goat anti-Mouse IgG [H&L] for detection.
pan Cytokeratin antibody [C-11]
GTX48825
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
Product group Antibodies
ReactivityHuman
TargetKRT6C
Overview
- SupplierGeneTex
- Product NameKeratin antibody [C11]
- Delivery Days Customer9
- Antibody SpecificityThis antibody reacts with keratin (56 kDa), keratin 17 (46 kDa), keratin 18 (45 kDa) and keratin 19 (40 kDa) derived from humans.
- Application Supplier NoteWB: 1:50-1:200. ICC/IF: 1:50-1:200. IHC-P: 1:50-1:200. IP: 1:100. ELISA: 1:5000-1:20000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDC-11
- Concentration1.3 mg/ml
- ConjugateUnconjugated
- Gene ID286887
- Target nameKRT6C
- Target descriptionkeratin 6C
- Target synonymsCK-6C; CK-6E; cytokeratin-6C; cytokeratin-6E; K6C; K6E; keratin 6C, type II; keratin 6E; keratin K6h; keratin, type II cytoskeletal 6C; KRT6E; PPKNEFD; type-II keratin Kb12
- HostMouse
- IsotypeIgG1
- Protein IDP48668
- Protein NameKeratin, type II cytoskeletal 6C
- Scientific DescriptionCytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (i.e. skin) and non-keratinizing cells (i.e. mesothelial)
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Extracellular vesicle analysis of plasma allows differential diagnosis of atypical pancreatic serous cystadenoma.Read more
- Multiparametric Circulating Tumor Cell Analysis to Select Targeted Therapies for Breast Cancer Patients. Franken A et al., 2021 Nov 29, Cancers (Basel)Read more
- Fabrication and Characterization of 3D Bioprinted Triple-layered Human Alveolar Lung Models. Ng WL et al., 2021, Int J BioprintRead more
- Human intestinal tissue-resident memory T cells comprise transcriptionally and functionally distinct subsets. FitzPatrick MEB et al., 2021 Jan 19, Cell RepRead more
- Acute kidney injury is associated with increased levels of circulating microvesicles in patients with decompensated cirrhosis. Campello E et al., 2021 Jul, Dig Liver DisRead more
- Tracking the expression of therapeutic protein targets in rare cells by antibody-mediated nanoparticle labelling and magnetic sorting. Labib M et al., 2021 Jan, Nat Biomed EngRead more
- Detection of ESR1 Mutations in Single Circulating Tumor Cells on Estrogen Deprivation Therapy but Not in Primary Tumors from Metastatic Luminal Breast Cancer Patients. Franken A et al., 2020 Jan, J Mol DiagnRead more
- Neutrophils escort circulating tumour cells to enable cell cycle progression. Szczerba BM et al., 2019 Feb, NatureRead more
- Single-cell mRNA cytometry via sequence-specific nanoparticle clustering and trapping. Labib M et al., 2018 May, Nat ChemRead more
- Isolation of Phenotypically Distinct Cancer Cells Using Nanoparticle-Mediated Sorting. Green BJ et al., 2017 Jun 21, ACS Appl Mater InterfacesRead more