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ProductsBack/KHDRBS1 Antibody
Western Blot analysis of COLO205 cells using Sam 68 Polyclonal Antibody
Western Blot analysis of COLO205 cells using Sam 68 Polyclonal Antibody
Western Blot analysis of COLO205 cells using Sam 68 Polyclonal Antibody

KHDRBS1 Antibody

CSB-PA004046
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetKHDRBS1
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Overview

  • Supplier
    Cusabio
  • Product Name
    KHDRBS1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10657
  • Target name
    KHDRBS1
  • Target description
    KH RNA binding domain containing, signal transduction associated 1
  • Target synonyms
    Sam68, p62, p68, KH domain-containing, RNA-binding, signal transduction-associated protein 1, GAP-associated tyrosine phosphoprotein p62 (Sam68), KH domain containing, RNA binding, signal transduction associated 1, p21 Ras GTPase-activating protein-associated p62, src-associated in mitosis 68 kDa protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ07666
  • Protein Name
    KH domain-containing, RNA-binding, signal transduction-associated protein 1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Sam68/KHDRBS1 using anti-Sam68/KHDRBS1 antibody (A01717-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human Daudi whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Sam68/KHDRBS1 antigen affinity purified polyclonal antibody (Catalog # A01717-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Sam68/KHDRBS1 at approximately 68 kDa. The expected band size for Sam68/KHDRBS1 is at 48 kDa.
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