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Western Blot analysis of Hela cells using FBP2 Polyclonal Antibody
Western Blot analysis of Hela cells using FBP2 Polyclonal Antibody
Western Blot analysis of Hela cells using FBP2 Polyclonal Antibody

KHSRP Antibody

CSB-PA002508
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetKHSRP
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Overview

  • Supplier
    Cusabio
  • Product Name
    KHSRP Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8570
  • Target name
    KHSRP
  • Target description
    KH-type splicing regulatory protein
  • Target synonyms
    FBP2, FUBP2, KSRP, p75, far upstream element-binding protein 2, FUSE-binding protein 2, epididymis secretory sperm binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92945
  • Protein Name
    Far upstream element-binding protein 2
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of KHSRP using anti-KHSRP antibody (A02770-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human Caco-2 whole cell lysates, Lane 7: human U-937 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KHSRP antigen affinity purified polyclonal antibody (Catalog # A02770-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KHSRP at approximately 85-90 kDa. The expected band size for KHSRP is at 73 kDa.
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