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Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-2: A431 cells, lovo cells, Primary antibody: CSB-PA980052(KIAA1524 Antibody) at dilution 1/597, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 second
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-2: A431 cells, lovo cells, Primary antibody: CSB-PA980052(KIAA1524 Antibody) at dilution 1/597, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 second
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-2: A431 cells, lovo cells, Primary antibody: CSB-PA980052(KIAA1524 Antibody) at dilution 1/597, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 second

KIAA1524 Antibody

CSB-PA980052
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetCIP2A
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Overview

  • Supplier
    Cusabio
  • Product Name
    KIAA1524 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57650
  • Target name
    CIP2A
  • Target description
    cellular inhibitor of PP2A
  • Target synonyms
    KIAA1524, NOCIVA, p90, protein CIP2A, cancerous inhibitor of PP2A, cancerous inhibitor of protein phosphatase 2A, cell proliferation regulating inhibitor of protein phosphatase 2A, p90 autoantigen
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TCG1
  • Protein Name
    Protein CIP2A
  • Scientific Description
    Protein CIP2A also known as cancerous inhibitor of PP2A (CIP2A) is a protein that in humans is encoded by the KIAA1524 gene. Protein phosphatase 2A (PP2A) is a cellular tumor suppressor which inhibits cell proliferation and transformation of normal cells to malignant cancer cells. Inhibition of PP2A activity is a prerequisite for human cell transformation. However, the in vivo mechanisms by which PP2A activity is inhibited in spontaneously transformed human cancer cells have been unclear.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of KIAA1524 using anti-KIAA1524 antibody (PB9714). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Mouse Testis Tissue Lysate at 50ug, Lane 2: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIAA1524 antigen affinity purified polyclonal antibody (Catalog # PB9714) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIAA1524 at approximately 90 kDa. The expected band size for KIAA1524 is at 102 kDa.
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