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KIAA1754L / ITPRIPL1 Antibody (aa43-71)

LS-C165340
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetITPRIPL1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    KIAA1754L / ITPRIPL1 Antibody (aa43-71)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID150771
  • Target name
    ITPRIPL1
  • Target description
    ITPRIP like 1
  • Target synonyms
    inositol 1,4,5-triphosphate receptor-interacting protein-like 1; inositol 1,4,5-trisphosphate receptor interacting protein-like 1; inositol 1,4,5-trisphosphate receptor-interacting protein-like 1; KIAA1754L
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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The Binding Activity of Macaca fascicularis ITPRIPL1 with Anti-ITPRIPL1 recombinant antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis ITPRIPL1 (CSB-MP6125MOV) at 2 microg/mL can bind Anti-ITPRIPL1 recombinant antibody. The EC50 is 3.759-4.428 ng/mL.
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Figure 1. Western blot analysis of ITPRIPL1 using anti-ITPRIPL1 antibody (A17509-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITPRIPL1 antigen affinity purified polyclonal antibody (Catalog # A17509-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITPRIPL1 at approximately 70 kDa. The expected band size for ITPRIPL1 is at 63 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetITPRIPL1
  • SizePrice