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KIF2A Antibody, FITC conjugated

CSB-PA012332LC01HU
Cusabio
ReactivityHuman
Product group Antibodies
TargetKIF2A
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Overview

  • Supplier
    Cusabio
  • Product Name
    KIF2A Antibody, FITC conjugated
  • Delivery Days Customer
    20
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    FITC
  • Gene ID3796
  • Target name
    KIF2A
  • Target description
    kinesin family member 2A
  • Target synonyms
    CDCBM3, HK2, KIF2, kinesin-like protein KIF2A, Kinesin, heavy chain, 2, kinesin heavy chain member 2A, kinesin-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00139
  • Protein Name
    Kinesin-like protein KIF2A
  • Scientific Description
    Plus end-directed microtubule-dependent motor required for normal brain development. May regulate microtubule dynamics during axonal growth. Required for normal progression through mitosis. Required for normal congress of chromosomes at the metaphase plate. Required for normal spindle dynamics during mitosis. Promotes spindle turnover. Implicated in formation of bipolar mitotic spindles. Has microtubule depolymerization activity.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of KIF2A using anti-KIF2A antibody (A03979-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat NRK whole cell lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIF2A antigen affinity purified polyclonal antibody (Catalog # A03979-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIF2A at approximately 110 kDa. The expected band size for KIF2A is at 110 kDa.
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ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse, Rat
TargetKIF2A
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TargetKIF2A
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Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with KIF2A Polyclonal Antibody, Unconjugated (bs-17055R) at 1:200 overnight at 4°C, DAB staining.
Product group Antibodies
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ReactivityBovine, Canine, Chicken, Equine, Human, Mouse, Porcine, Rat, Sheep
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KIF2A antibodyGTX130808
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