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ProductsBack/KIF7 Antibody (clone 3I4)

KIF7 Antibody (clone 3I4)

LS-C767768
LifeSpan BioSciences
ApplicationsImmunoFluorescence, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetKIF7
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    KIF7 Antibody (clone 3I4)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ImmunoFluorescence, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IF (1:50 - 1:200), IHC-P Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3I4
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID374654
  • Target name
    KIF7
  • Target description
    kinesin family member 7
  • Target synonyms
    ACLS; AGBK; EQYK340; HLS2; JBTS12; kinesin-like protein KIF7; UNQ340
  • Host
    Mouse
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KIF7 using anti-KIF7 antibody (A04321-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human A375 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIF7 antigen affinity purified polyclonal antibody (Catalog # A04321-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIF7 at approximately 151 kDa. The expected band size for KIF7 is at 151 kDa.
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