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KIFC1 Antibody, Biotin conjugated

CSB-PA871587LD01HU
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityHuman
TargetKIFC1
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Overview

  • Supplier
    Cusabio
  • Product Name
    KIFC1 Antibody, Biotin conjugated
  • Delivery Days Customer
    20
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Biotin
  • Gene ID3833
  • Target name
    KIFC1
  • Target description
    kinesin family member C1
  • Target synonyms
    HSET, KNSL2, kinesin-like protein KIFC1, kinesin-like 2, kinesin-like protein 2, kinesin-related protein HSET
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BW19
  • Protein Name
    Kinesin-like protein KIFC1
  • Scientific Description
    Minus end-directed microtubule-dependent motor required for bipolar spindle formation (PubMed:15843429). May contribute to movement of early endocytic vesicles (By similarity). Regulates cilium formation and structure (By similarity).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Immunofluorescence staining of Hela cells with CSB-PA871587LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Figure 1. Western blot analysis of KIFC1 using anti-KIFC1 antibody (A05325-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIFC1 antigen affinity purified polyclonal antibody (Catalog # A05325-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIFC1 at approximately 74 kDa. The expected band size for KIFC1 is at 74 kDa.
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