Belgium

Luxembourg

Netherlands

Kits

cDNA synthesis

Other Application

Applications

Euroclone

Jena Bioscience

MyBioSource

OriGene

Supplier

Research Use Only

Certification

Business Unit

RNT-201 | HighYield T7 P&L RNA Synthesis Kit, RNA synthesis via in vitro transcription with a modified T7 RNA Polymerase (P266L).

HighYield T7 P&L RNA Synthesis Kit, RNA... | RNT-201 | Jena Bioscience

HighYield T7 P&L RNA Synthesis Kit, RNA synthesis via in vitro transcription with a modified T7 RNA Polymerase (P266L)

Molecular Biology

HighYield T7 P&L RNA Synthesis Kit is designed to produce large amounts of RNA via in vitro transcription with a modified T7 RNA Polymerase. Proline 266 has been replaced by leucine (P266L) which has been associated with decreased abortive transcription[1].increased 5 homogeneity of transcripts synthesized from A-initiating phi2.5 promoter[2]increased 5 incorporation efficiency of GTP analogs[3] The resulting RNA can subsequently be used for a variety of applications such as microinjection, transfection or in vitro translation experiments, structure/function studies or probes for in situ hybridization and Northern Blot experiments. One kit contains sufficient reagents for 50 reactions of 20 microl each (7.5 mM each NTP). A 20 microl reaction yields about 140-160 microg RNA after 30 min incubation (1 microg T7 control template, 1.4 kb RNA transcript). Yields may however vary depending on the template (promoter design, sequence length, secondary structure formation).

MBS9135688 | mRNA-seq Lib Prep Kit | Packing: 24  reactions | MyBioSource available at Bio-Connect

mRNA-seq Lib Prep Kit | MBS9135688 | MyBioSource

mRNA-seq Lib Prep Kit

MBS9141671 | cDNA First Strand Synthesis | Packing: 50  reactions | MyBioSource available at Bio-Connect

cDNA First Strand Synthesis | MBS9141671 | MyBioSource

cDNA First Strand Synthesis

Website

Tech support

Our company

Our team

Divisions

Vacatures

Events

Promotions

About us

Contact

Our people

Antibodies

Chemicals

Proteins / Signaling Molecules

Assays

(Cell) Culture

Products

Our Company

Suppliers

News

Bio-Connect

Biotech, Pharma & CRO

Academia

Diagnostics

Environment

Food

Veterinary

Industries

Disclaimer

Privacy Statement

Terms and conditions

Are you looking for specific products, alternatives, documentation or need assistance with solving product related issues? Let our in-house technical support team help you.

We support your research

<p>For 15 years, we connect life science researchers specialized in the import and distribution of life science reagents from global suppliers.</p>

Support your research

DNA / RNA / Vectors

Cell Culture

Genome Editing and Engineering

Lysates / Extracts

Expression

Labware / Accesories

Body Products

Technique Specific Products

Arrays / Slides

Call +31 (0)26 326 44 50

Kits

Didn't find what you were looking for?