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KLC1 Antibody (YA2363, Rabbit)

HY-P82618
MedChem Express
TargetKLC1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    KLC1 Antibody (YA2363, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID3831
  • Target name
    KLC1
  • Target description
    kinesin light chain 1
  • Target synonyms
    KLC, KNS2, KNS2A, kinesin light chain 1, kinesin 2 60/70kDa, medulloblastoma antigen MU-MB-2.50
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    KLC1 Antibody (YA2363) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2363), targeting KLC1, with a predicted molecular weight of 65 kDa (observed band size: 65 kDa). KLC1 Antibody (YA2363) can be used for WB, IHC-P, ICC/IF, IP, FC experiment in human, mouse, rat background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of KLC1 using anti-KLC1 antibody (A04116-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human COLO-320 whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KLC1 antigen affinity purified polyclonal antibody (Catalog # A04116-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KLC1 at approximately 65-75 kDa. The expected band size for KLC1 is at 65-75 kDa.
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