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WB analysis of various sample lysates using GTX66176 KLF10 antibody. Dilution : 1:3000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX66176 KLF10 antibody. Dilution : 1:3000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX66176 KLF10 antibody. Dilution : 1:3000 Loading : 25microg per lane

KLF10 antibody

GTX66176
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetKLF10
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Overview

  • Supplier
    GeneTex
  • Product Name
    KLF10 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7071
  • Target name
    KLF10
  • Target description
    Kruppel like factor 10
  • Target synonyms
    early growth response-alpha; EGRA; EGR-alpha; Krueppel-like factor 10; TGFB-inducible early growth response protein 1; TIEG; TIEG1; transforming growth factor-beta-inducible early growth response protein 1; zinc finger transcription factor TIEG
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13118
  • Protein Name
    Krueppel-like factor 10
  • Scientific Description
    This gene encodes a member of a family of proteins that feature C2H2-type zinc finger domains. The encoded protein is a transcriptional repressor that acts as an effector of transforming growth factor beta signaling. Activity of this protein may inhibit the growth of cancers, particularly pancreatic cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KLF10 using anti-KLF10 antibody (A03419-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KLF10 antigen affinity purified polyclonal antibody (Catalog # A03419-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KLF10 at approximately 55 kDa. The expected band size for KLF10 is at 53 kDa.
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ICC/IF analysis of SiHa cells using GTX04519 KLF10 antibody. Green : Primary antibody Blue : DAPI Antigen retrieval : Enzyme antigen retrieval was performed. Dilution : 5 microg/mL
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