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KLF15 antibody

70R-18141
Biosynth
Product group Antibodies
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Overview

  • Supplier
    Biosynth
  • Product Name
    KLF15 antibody
  • Delivery Days Customer
    11
  • Certification
    Research Use Only
  • UNSPSC
    12352203

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The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA103566(KLF15 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200)
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KLF15 antibody [N1], N-term detects KLF15 protein by western blot analysis. Non-transfected (-), KLF4-transfected (+), KLF12-transfected (+) and KLF15-transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF15 antibody [N1], N-term (GTX104839) diluted at 1:1000.
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Figure 1. Western blot analysis of KLF15 using anti-KLF15 antibody (A03661-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KLF15 antigen affinity purified polyclonal antibody (Catalog # A03661-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KLF15 at approximately 44 kDa. The expected band size for KLF15 is at 44 kDa.
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