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ProductsBack/KLHL12 (D-1): m-IgG Fc BP-HRP Bundle

KLHL12 (D-1): m-IgG Fc BP-HRP Bundle

SC-526398
Santa Cruz Biotechnology
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
TargetKLHL12
1 kit
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    KLHL12 (D-1): m-IgG Fc BP-HRP Bundle
  • Delivery Days Customer
    5
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID59349
  • Target name
    KLHL12
  • Target description
    kelch like family member 12
  • Target synonyms
    C3IP1; CUL3-interacting protein 1; DKIR; DKIR homolog; kelch-like protein 12; kelch-like protein C3IP1
  • Host
    Mouse
  • Isotype
    IgG2b
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KLHL12 using anti-KLHL12 antibody (A08568-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KLHL12 antigen affinity purified polyclonal antibody (Catalog # A08568-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KLHL12 at approximately 63 kDa. The expected band size for KLHL12 is at 63 kDa.
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