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ProductsBack/KLK2 / Kallikrein 2 Antibody (aa25-261, clone OTI5D6, Carrier-free)

KLK2 / Kallikrein 2 Antibody (aa25-261, clone OTI5D6, Carrier-free)

LS-C789429
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetKLK2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    KLK2 / Kallikrein 2 Antibody (aa25-261, clone OTI5D6, Carrier-free)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:150), WB (1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI5D6
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3817
  • Target name
    KLK2
  • Target description
    kallikrein related peptidase 2
  • Target synonyms
    KLK2A2, hGK-1, hK2, kallikrein-2, glandular kallikrein 2, glandular kallikrein-1, kallikrein 2, prostatic, tissue kallikrein-2
  • Host
    Mouse
  • Isotype
    IgG2b
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Kallikrein 2 using anti-Kallikrein 2 antibody (A03879). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MCF-7 cell lysate, Lane 2: human COLO-320 cell lysate, Lane 3: human SK-OV-3 cell lysate, Lane 4: human HepG2 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kallikrein 2 antigen affinity purified polyclonal antibody (Catalog # A03879) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kallikrein 2 at approximately 21KD. The expected band size for Kallikrein 2 is at 28KD.
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