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Klk7 Antibody, FITC conjugated

CSB-PA012458LC01RA
Cusabio
ReactivityRat
Product group Antibodies
TargetKlk1
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Overview

  • Supplier
    Cusabio
  • Product Name
    Klk7 Antibody, FITC conjugated
  • Delivery Days Customer
    20
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    FITC
  • Gene ID24523
  • Target name
    Klk1
  • Target description
    kallikrein 1
  • Target synonyms
    KALA, Kal, Klk1c7, Klk1l, Klk5, Klk7, Klna3, RATKALA, RGK-7, RSKG-7, kallikrein 1, Kallikrein 1, renal/pancreas/salivary, esterase B, glandular kallikrein-7, submandibular/renal, kallikrein 1-like peptidase, kallikrein 1-related peptidase C7, kallikrein 5, kallikrein 7, kallikrein a3, kallikrein-related protein K1, proteinase A, renal kallikrein, tissue kallikrein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP36373
  • Protein Name
    Glandular kallikrein-7, submandibular/renal
  • Scientific Description
    Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. Predominant kallikrein protein in the kidney.
  • Reactivity
    Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Klk7 using anti-Klk7 antibody (A02739-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Klk7 antigen affinity purified polyclonal antibody (Catalog # A02739-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Klk7 at approximately 38 kDa. The expected band size for Klk7 is at 28 kDa.
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