Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Klotho Polyclonal Antibody, Unconjugated (bs-2925R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Klotho Polyclonal Antibody, Unconjugated (bs-2925R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Klotho Polyclonal Antibody
BS-2925R
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetKL
Overview
- SupplierBioss
- Product NameKlotho Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(WB=1:200-2000), IHC-P(IHC-P=1:100-500), IHC-F(IHC-F=1:100-500), IF(IF=1:100-500)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID9365
- Target nameKL
- Target descriptionklotho
- Target synonymsHFTC3, KLA, klotho, alpha-klotho
- HostRabbit
- IsotypeIgG
- Protein IDQ9UEF7
- Protein NameKlotho
- Storage Instruction-20°C
- UNSPSC12352203
References
- Prud'homme GJ, Glinka Y, Kurt M, et al. The anti-aging protein Klotho is induced by GABA therapy and exerts protective and stimulatory effects on pancreatic beta cells. Biochem Biophys Res Commun. 2017,493(4):1542-1547. doi: 10.1016/j.bbrc.2017.10.029Read this paper
- Ni W, Fang Y, Xie L, et al. Adipose-Derived Mesenchymal Stem Cells Transplantation Alleviates Renal Injury in Streptozotocin-Induced Diabetic Nephropathy. J Histochem Cytochem. 2015,63(11):842-53. doi: 10.1369/0022155415599039Read this paper
Datasheet
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