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ProductsBack/[KO Validated] 14-3-3 gamma Antibody

[KO Validated] 14-3-3 gamma Antibody

CAB18060
Assay Genie
TargetGGCX
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    [KO Validated] 14-3-3 gamma Rabbit pAb
  • Delivery Days Customer
    9
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID2677
  • Target name
    GGCX
  • Target description
    gamma-glutamyl carboxylase
  • Target synonyms
    peptidyl-glutamate 4-carboxylase; vitamin K-dependent gamma-carboxylase; VKCFD1
  • Host
    Rabbit
  • Protein IDP38435
  • Protein Name
    Vitamin K-dependent gamma-carboxylase
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GGCX using anti-GGCX antibody (A02643-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GGCX antigen affinity purified polyclonal antibody (Catalog # A02643-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GGCX at approximately 88 kDa. The expected band size for GGCX is at 88 kDa.
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