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[KO Validated] LC3B Antibody

CAB11282
Assay Genie
TargetNCOA1
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    [KO Validated] LC3B Rabbit pAb
  • Delivery Days Customer
    9
  • Applications Supplier
    WB,IHC,IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID8648
  • Target name
    NCOA1
  • Target description
    nuclear receptor coactivator 1
  • Target synonyms
    F-SRC-1, KAT13A, RIP160, SRC1, bHLHe42, bHLHe74, nuclear receptor coactivator 1, Hin-2 protein, class E basic helix-loop-helix protein 74, renal carcinoma antigen NY-REN-52, steroid receptor coactivator-1
  • Host
    Rabbit
  • Protein IDQ15788
  • Protein Name
    Nuclear receptor coactivator 1
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of KAT13A/SRC1 using anti-KAT13A/SRC1 antibody (RP1056). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: SK-OV-3 whole cell lysates, Lane 2: SGC-7901 whole cell lysates, Lane 3: Jurkat whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KAT13A/SRC1 antigen affinity purified polyclonal antibody (Catalog # RP1056) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KAT13A/SRC1 at approximately 157KD. The expected band size for KAT13A/SRC1 is at 157KD.
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