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ProductsBack/KPNA2 / Importin Alpha 1 Antibody (aa2-132, clone 12K121)

KPNA2 / Importin Alpha 1 Antibody (aa2-132, clone 12K121)

LS-C182895
LifeSpan BioSciences
ApplicationsImmunoCytoChemistry
Product group Antibodies
TargetKPNA2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    KPNA2 / Importin Alpha 1 Antibody (aa2-132, clone 12K121)
  • Delivery Days Customer
    23
  • Applications
    ImmunoCytoChemistry
  • Applications Supplier
    ICC (8 - 25 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    12K121
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3838
  • Target name
    KPNA2
  • Target description
    karyopherin subunit alpha 2
  • Target synonyms
    importin subunit alpha-1; importin subunit alpha-2; importin-alpha-P1; IPOA1; karyopherin alpha 2 (RAG cohort 1, importin alpha 1); pendulin; QIP2; RAG cohort protein 1; RCH1; SRP1alpha; SRP1-alpha
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KPNA2 using anti-KPNA2 antibody (A01776-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate,Lane 2: human HepG2 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KPNA2 antigen affinity purified polyclonal antibody (Catalog # A01776-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KPNA2 at approximately 58KD. The expected band size for KPNA2 is at 58KD.
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