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Western Blot Positive WB detected in: MCF-7 whole cell lysate, Jurkat whole cell lysate All lanes: KTN1 antibody at 3.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 157, 150, 151, 153 kDa Observed band size: 157 kDa
Western Blot Positive WB detected in: MCF-7 whole cell lysate, Jurkat whole cell lysate All lanes: KTN1 antibody at 3.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 157, 150, 151, 153 kDa Observed band size: 157 kDa
Western Blot Positive WB detected in: MCF-7 whole cell lysate, Jurkat whole cell lysate All lanes: KTN1 antibody at 3.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 157, 150, 151, 153 kDa Observed band size: 157 kDa

KTN1 Antibody

CSB-PA773030LA01HU
Cusabio
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetKTN1
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Overview

  • Supplier
    Cusabio
  • Product Name
    KTN1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3895
  • Target name
    KTN1
  • Target description
    kinectin 1
  • Target synonyms
    CG1; CG-1 antigen; kinectin; kinesin receptor; KNT; MU-RMS-40.19
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ86UP2
  • Protein Name
    Kinectin
  • Scientific Description
    Receptor for kinesin thus involved in kinesin-driven vesicle motility. Accumulates in integrin-based adhesion complexes (IAC) upon integrin aggregation by fibronectin.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Kinectin 1/KTN1 using anti-Kinectin 1/KTN1 antibody (A05071-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey COS-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat ovary tissue lysates, Lane 4: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kinectin 1/KTN1 antigen affinity purified polyclonal antibody (Catalog # A05071-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kinectin 1/KTN1 at approximately 160 kDa. The expected band size for Kinectin 1/KTN1 is at 160 kDa.
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