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WB analysis of 293 cell lysate (35ug/lane) using GTX53679 Kv1.4 antibody.
WB analysis of 293 cell lysate (35ug/lane) using GTX53679 Kv1.4 antibody.
WB analysis of 293 cell lysate (35ug/lane) using GTX53679 Kv1.4 antibody.

Kv1.4 antibody

GTX53679
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetKCNA4
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Overview

  • Supplier
    GeneTex
  • Product Name
    Kv1.4 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3739
  • Target name
    KCNA4
  • Target description
    potassium voltage-gated channel subfamily A member 4
  • Target synonyms
    cardiac potassium channel; fetal skeletal muscle potassium channel; HBK4; HK1; HPCN2; HUKII; KCNA4L; KCNA8; KV1.4; MCIDDS; PCN2; potassium channel 2; potassium channel, voltage gated shaker related subfamily A, member 4; potassium voltage-gated channel subfamily A member 4; rapidly inactivating potassium channel; shaker-related potassium channel Kv1.4; type A potassium channel; voltage-gated K(+) channel HuKII; voltage-gated potassium channel HBK4; voltage-gated potassium channel HK1; voltage-gated potassium channel subunit Kv1.4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP22459
  • Protein Name
    Potassium voltage-gated channel subfamily A member 4
  • Scientific Description
    Potassium channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shaker-related subfamily. This member contains six membrane-spanning domains with a shaker-type repeat in the fourth segment. It belongs to the A-type potassium current class, the members of which may be important in the regulation of the fast repolarizing phase of action potentials in heart and thus may influence the duration of cardiac action potential.[provided by RefSeq, Mar 2011]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Kv1.4 using anti-Kv1.4 antibody (PB9807). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: HELA Whole Cell Lysate, Lane 2: COLO320 Whole Cell Lysate, Lane 3: HT1080 Whole Cell Lysate, Lane 4: PANC Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kv1.4 antigen affinity purified polyclonal antibody (Catalog # PB9807) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kv1.4 at approximately 73 kDa. The expected band size for Kv1.4 is at 73 kDa.
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