L-Homopropargylglycine (L-HPG) [942518-19-6]

Jena Bioscience

L-Homopropargylglycine (L-HPG) [942518-19-6]

5

942518-19-6

Research Use Only

C6H9NO2 * HCl

163.60 g/mol (HCl salt)

L-Homopropargylglycine (L-HPG) provides a non-radioactive alternative to analyze the global protein synthesis in cell culture. It is cell-permeable and randomly incorporated instead of methionine during translation[1,2,3]. The resulting alkyne-labeled full-length proteins can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks or a fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging. Presolski et al.[4] and Hong et al.[5] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.

[C@@H](C(=O)O)(CCC#C)N.Cl

-20°C

12352200