IHC-P analysis of human liver tissue using GTX84241 L1CAM antibody [2C7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
![FACS analysis of HeLa cells using GTX84241 L1CAM antibody [2C7]. Red : Primary antibody Blue : Negative control antibody](https://www.genetex.com/upload/website/prouct_img/normal/GTX84241/GTX84241_333_FACS_w_23061420_107.webp "FACS analysis of HeLa cells using GTX84241 L1CAM antibody [2C7]. Red : Primary antibody Blue : Negative control antibody")
![FACS analysis of HEK293T cells transfected with either L1CAM plasmid(Red) or empty vector control plasmid(Blue) using GTX84241 L1CAM antibody [2C7].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84241/GTX84241_334_FACS_w_23061420_146.webp "FACS analysis of HEK293T cells transfected with either L1CAM plasmid(Red) or empty vector control plasmid(Blue) using GTX84241 L1CAM antibody [2C7].")
![IP analysis of DDDDK tagged L1CAM overexpressed HEK293T lysate using GTX84241 L1CAM antibody [2C7].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate](https://www.genetex.com/upload/website/prouct_img/normal/GTX84241/GTX84241_3359_IP_w_23061420_558.webp "IP analysis of DDDDK tagged L1CAM overexpressed HEK293T lysate using GTX84241 L1CAM antibody [2C7].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate")
![WB analysis of various cell lines using GTX84241 L1CAM antibody [2C7]. Loading : 35 ug per lane Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX84241/GTX84241_3511_WB_w_23061420_631.webp "WB analysis of various cell lines using GTX84241 L1CAM antibody [2C7]. Loading : 35 ug per lane Dilution : 1:200")
![WB analysis of HEK293T cells transfected with L1CAM plasmid (Right) or empty vector (Left) for 48 hrs using GTX84241 L1CAM antibody [2C7]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84241/GTX84241_4204_WB_w_23061420_263.webp "WB analysis of HEK293T cells transfected with L1CAM plasmid (Right) or empty vector (Left) for 48 hrs using GTX84241 L1CAM antibody [2C7]. Loading : 5 ug per lane")
![ICC/IF analysis of COS7 cells transiently transfected with L1CAM plasmid using GTX84241 L1CAM antibody [2C7].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84241/GTX84241_989_ICCIF_w_23061420_766.webp "ICC/IF analysis of COS7 cells transiently transfected with L1CAM plasmid using GTX84241 L1CAM antibody [2C7].")
![ICC/IF analysis of HeLa cells using GTX84241 L1CAM antibody [2C7].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84241/GTX84241_990_ICCIF_w_23061420_350.webp "ICC/IF analysis of HeLa cells using GTX84241 L1CAM antibody [2C7].")
IHC-P analysis of human liver tissue using GTX84241 L1CAM antibody [2C7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
L1CAM antibody [2C7]
GTX84241
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Monkey
TargetL1CAM
Overview
- SupplierGeneTex
- Product NameL1CAM antibody [2C7] - Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:2000. ICC/IF: 1:50-1:100. IHC-P: 1:50. FACS: 1:100. IP: 2-4ug/mg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID2C7
- Concentration0.46 mg/ml
- ConjugateUnconjugated
- Gene ID3897
- Target nameL1CAM
- Target descriptionL1 cell adhesion molecule
- Target synonymsantigen identified by monoclonal antibody R1; CAML1; CD171; HSAS; HSAS1; MASA; MIC5; N-CAML1; N-CAM-L1; NCAM-L1; neural cell adhesion molecule L1; S10; SPG1
- HostMouse
- IsotypeIgG1
- Protein IDP32004
- Protein NameNeural cell adhesion molecule L1
- Scientific DescriptionCell adhesion molecule with an important role in the development of the nervous system. Involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. Binds to axonin on neurons.
- ReactivityHuman, Monkey
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203