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Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA729208LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA729208LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA729208LA01HU at dilution of 1:100

L1TD1 Antibody

CSB-PA729208LA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetL1TD1
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Overview

  • Supplier
    Cusabio
  • Product Name
    L1TD1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54596
  • Target name
    L1TD1
  • Target description
    LINE1 type transposase domain containing 1
  • Target synonyms
    ECAT11, LINE-1 type transposase domain-containing protein 1, ES cell-associated protein 11, LINE-1 type transposase domain containing 1, embryonic stem cell-associated transcript 11
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5T7N2
  • Protein Name
    LINE-1 type transposase domain-containing protein 1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of L1TD1 using anti-L1TD1 antibody (A12503-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: rat testis lysates, Lane 3: rat C6 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates, Lane 7: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-L1TD1 antigen affinity purified polyclonal antibody (Catalog # A12503-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for L1TD1 at approximately 120 kDa. The expected band size for L1TD1 is at 120 kDa.
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