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L3MBTL2 Antibody (aa31-80, Biotin)

LS-C425659
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetL3MBTL2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    L3MBTL2 Antibody (aa31-80, Biotin)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    Western Blot
  • Applications Supplier
    WB The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Biotin
  • Estimated Purity
    ...
  • Gene ID83746
  • Target name
    L3MBTL2
  • Target description
    L3MBTL histone methyl-lysine binding protein 2
  • Target synonyms
    H-l(3)mbt-l; H-l(3)mbt-like protein 2; l(3)mbt-like 2; L(3)mbt-like protein 2; L3MBT; L3MBTL2, polycomb repressive complex 1 subunit; lethal(3)malignant brain tumor-like protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of L3MBTL2 using anti-L3MBTL2 antibody (A08416-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-L3MBTL2 antigen affinity purified polyclonal antibody (Catalog # A08416-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for L3MBTL2 at approximately 79 kDa. The expected band size for L3MBTL2 is at 79 kDa.
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