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Western Blot Positive WB detected in: K562 whole cell lysate, Rat heart tissue All lanes: L3MBTL2 antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 80, 70 kDa Observed band size: 70 kDa
Western Blot Positive WB detected in: K562 whole cell lysate, Rat heart tissue All lanes: L3MBTL2 antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 80, 70 kDa Observed band size: 70 kDa
Western Blot Positive WB detected in: K562 whole cell lysate, Rat heart tissue All lanes: L3MBTL2 antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 80, 70 kDa Observed band size: 70 kDa

L3MBTL2 Antibody

CSB-PA839279LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Rat
TargetL3MBTL2
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Overview

  • Supplier
    Cusabio
  • Product Name
    L3MBTL2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID83746
  • Target name
    L3MBTL2
  • Target description
    L3MBTL histone methyl-lysine binding protein 2
  • Target synonyms
    H-l(3)mbt-l; H-l(3)mbt-like protein 2; l(3)mbt-like 2; L(3)mbt-like protein 2; L3MBT; L3MBTL2, polycomb repressive complex 1 subunit; lethal(3)malignant brain tumor-like protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ969R5
  • Protein Name
    Lethal(3)malignant brain tumor-like protein 2
  • Scientific Description
    Putative Polycomb group (PcG) protein. PcG proteins maintain the transcriptionally repressive state of genes, probably via a modification of chromatin, rendering it heritably changed in its expressibility. Its association with a chromatin-remodeling complex suggests that it may contribute to prevent expression of genes that trigger the cell into mitosis. Binds to monomethylated and dimethylated Lys-20 on histone H4. Binds histone H3 peptides that are monomethylated or dimethylated on Lys-4, Lys-9 or Lys-27.
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of L3MBTL2 using anti-L3MBTL2 antibody (A08416-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-L3MBTL2 antigen affinity purified polyclonal antibody (Catalog # A08416-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for L3MBTL2 at approximately 79 kDa. The expected band size for L3MBTL2 is at 79 kDa.
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