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ProductsBack/LAD1 Antibody (aa1-192)

LAD1 Antibody (aa1-192)

LS-C374554
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetLAD1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    LAD1 Antibody (aa1-192)
  • Delivery Days Customer
    23
  • Application Supplier Note
    ELISA 1:100-1:5000; ICC 1:50-500; WB 1:50-400;
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:50 - 1:400) ELISA 1:100-1:5000; ICC 1:50-500; WB 1:50-400;
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3898
  • Target name
    LAD1
  • Target description
    ladinin 1
  • Target synonyms
    LadA, ladinin-1, lad-1, linear IgA bullous dermatosis antigen, linear IgA disease antigen homolog
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LAD1 using anti-LAD1 antibody (A07940). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LAD1 antigen affinity purified polyclonal antibody (Catalog # A07940) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LAD1 at approximately 65 kDa. The expected band size for LAD1 is at 57 kDa.
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