Immunohistochemical analysis of paraffin-embedded human lung cancer, using Laminin beta 3(GTX103736) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
![Laminin beta 3 antibody [C1C2], Internal detects Laminin beta 3 protein by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Laminin beta 3 stained by Laminin beta 3 antibody [C1C2], Internal (GTX103736) diluted at 1:500. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX103736/GTX103736_43118_20180314_ICC_IF_w_23060119_890.webp "Laminin beta 3 antibody [C1C2], Internal detects Laminin beta 3 protein by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Laminin beta 3 stained by Laminin beta 3 antibody [C1C2], Internal (GTX103736) diluted at 1:500. Blue: Hoechst 33342 staining.")
![Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Laminin beta 3 antibody [C1C2], Internal (GTX103736) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX103736/GTX103736_43118_20180216_WB_w_23060119_252.webp "Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Laminin beta 3 antibody [C1C2], Internal (GTX103736) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Immunohistochemical analysis of paraffin-embedded human lung cancer, using Laminin beta 3(GTX103736) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Laminin beta 3 antibody [C1C2], Internal
GTX103736
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetLAMB3
Overview
- SupplierGeneTex
- Product NameLaminin beta 3 antibody [C1C2], Internal
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.46 mg/ml
- ConjugateUnconjugated
- Gene ID3914
- Target nameLAMB3
- Target descriptionlaminin subunit beta 3
- Target synonymsAI1A, BM600-125KDA, JEB1A, JEB1B, LAM5, LAMNB1, laminin subunit beta-3, epiligrin subunit bata, kalinin B1 chain, kalinin subunit beta, kalinin-140kDa, laminin B1k chain, laminin S B3 chain, laminin beta 3, laminin, beta 3 (nicein (125kD), kalinin (140kD), BM600 (125kD)), laminin-5 subunit beta, nicein subunit beta, nicein-125kDa
- HostRabbit
- IsotypeIgG
- Protein IDQ13751
- Protein NameLaminin subunit beta-3
- Scientific DescriptionThe product encoded by this gene is a laminin that belongs to a family of basement membrane proteins. This protein is a beta subunit laminin, which together with an alpha and a gamma subunit, forms laminin-5. Mutations in this gene cause epidermolysis bullosa junctional Herlitz type, and generalized atrophic benign epidermolysis bullosa, diseases that are characterized by blistering of the skin. Multiple alternatively spliced transcript variants that encode the same protein have been found for this gene. [provided by RefSeq]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Uehara N, Kukita A, Kyumoto-Nakamura Y, et al. Osteoblast-derived Laminin-332 is a novel negative regulator of osteoclastogenesis in bone microenvironments. Lab Invest. 2017,97(10):1235-1244. doi: 10.1038/labinvest.2017.55Read this paper
Datasheet
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![Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Laminin beta 3 antibody [C1C3] (GTX110583) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX110583/GTX110583_40450_20170629_WB_w_23060500_165.webp)
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