Paraformaldehyde-fixed, paraffin embedded rat intestine tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Laminin Polyclonal Antibody, Unconjugated (bs-0821R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat intestine tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Laminin Polyclonal Antibody, Unconjugated (bs-0821R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining
Laminin subunit beta-1 Polyclonal Antibody
BS-0821R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetLAMB1
Overview
- SupplierBioss
- Product NameLaminin subunit beta-1 Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID3912
- Target nameLAMB1
- Target descriptionlaminin subunit beta 1
- Target synonymsCLM, LIS5, laminin subunit beta-1, laminin B1 chain, laminin, beta 1
- HostRabbit
- IsotypeIgG
- Protein IDP07942
- Protein NameLaminin subunit beta-1
- Storage Instruction-20°C
- UNSPSC12352203
References
- Wang Y, Liu S, Yan Y, et al. SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β cell signaling pathway. Cell Death Dis. 2019,10(11):852. doi: 10.1038/s41419-019-2049-4Read this paper
- Miyauchi Y, Yasuchika K, Fukumitsu K, et al. A novel three-dimensional culture system maintaining the physiological extracellular matrix of fibrotic model livers accelerates progression of hepatocellular carcinoma cells. Sci Rep. 2017,7(1):9827. doi: 10.1038/s41598-017-09391-yRead this paper
- Gong T, Zhao K, Liu X, et al. A Dynamically Tunable, Bioinspired Micropatterned Surface Regulates Vascular Endothelial and Smooth Muscle Cells Growth at Vascularization. Small. 2016,12(41):5769-5778. doi: 10.1002/smll.201601503Read this paper
Datasheet
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