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WB analysis of RAW264.7 cell lysates using GTX87286 LARG antibody. The lane on the right is blocked with the synthesized peptide.
WB analysis of RAW264.7 cell lysates using GTX87286 LARG antibody. The lane on the right is blocked with the synthesized peptide.
WB analysis of RAW264.7 cell lysates using GTX87286 LARG antibody. The lane on the right is blocked with the synthesized peptide.

LARG antibody

GTX87286
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetARHGEF12
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Overview

  • Supplier
    GeneTex
  • Product Name
    LARG antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500~1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23365
  • Target name
    ARHGEF12
  • Target description
    Rho guanine nucleotide exchange factor 12
  • Target synonyms
    LARG, PRO2792, rho guanine nucleotide exchange factor 12, KMT2A/ARHGEF12 fusion protein, Rho guanine nucleotide exchange factor (GEF) 12, leukemia-associated RhoGEF, leukemia-associated rho guanine nucleotide exchange factor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NZN5
  • Protein Name
    Rho guanine nucleotide exchange factor 12
  • Scientific Description
    Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli working through G protein-coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein has been observed to form a myeloid/lymphoid fusion partner in acute myeloid leukemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Nanda S, Calderon A, Sachan A, et al. Rho GTPase activity crosstalk mediated by Arhgef11 and Arhgef12 coordinates cell protrusion-retraction cycles. Nat Commun. 2023,14(1):8356. doi: 10.1038/s41467-023-43875-y
    Read this paper

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Figure 1. Western blot analysis of LARG/ARHGEF12 using anti-LARG/ARHGEF12 antibody (A06802-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LARG/ARHGEF12 antigen affinity purified polyclonal antibody (Catalog # A06802-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The si2093gnal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LARG/ARHGEF12 at approximately 220 kDa. The expected band size for LARG/ARHGEF12 is at 173 kDa.
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