More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/LARG antibody
WB analysis of RAW264.7 cell lysates using GTX87286 LARG antibody. The lane on the right is blocked with the synthesized peptide.
WB analysis of RAW264.7 cell lysates using GTX87286 LARG antibody. The lane on the right is blocked with the synthesized peptide.
WB analysis of RAW264.7 cell lysates using GTX87286 LARG antibody. The lane on the right is blocked with the synthesized peptide.

LARG antibody

GTX87286
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetARHGEF12
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    LARG antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500~1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23365
  • Target name
    ARHGEF12
  • Target description
    Rho guanine nucleotide exchange factor 12
  • Target synonyms
    LARG; leukemia-associated rho guanine nucleotide exchange factor; leukemia-associated RhoGEF; PRO2792; Rho guanine nucleotide exchange factor (GEF) 12; rho guanine nucleotide exchange factor 12
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NZN5
  • Protein Name
    Rho guanine nucleotide exchange factor 12
  • Scientific Description
    Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli working through G protein-coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein has been observed to form a myeloid/lymphoid fusion partner in acute myeloid leukemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Rho GTPase activity crosstalk mediated by Arhgef11 and Arhgef12 coordinates cell protrusion-retraction cycles.
    Read more

Related products

Western Blot analysis of RAW264.7 cells using LARG Polyclonal Antibody
Product group Antibodies
Cusabio
ARHGEF12 AntibodyCSB-PA008357
ApplicationsWestern Blot, ELISA
TargetARHGEF12
  • SizePrice
Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules and cells in glomeruli.
Product group Antibodies
Atlas Antibodies
Anti-ARHGEF12 AntibodyHPA018911
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
TargetARHGEF12
  • SizePrice
Product group Antibodies
Bethyl Laboratories
Rabbit anti-LARG Antibody, Affinity PurifiedA301-959A
ApplicationsWestern Blot
TargetARHGEF12
  • SizePrice
Anti-ARHGEF12 Antibody
Product group Antibodies
Antibodies.com
Anti-ARHGEF12 AntibodyA96345
ApplicationsWestern Blot, ELISA
  • SizePrice
Product group Antibodies
References
Bioss Antibodies
LARG Polyclonal AntibodyBS-4213R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetARHGEF12
  • SizePrice
Figure 1. Western blot analysis of LARG/ARHGEF12 using anti-LARG/ARHGEF12 antibody (A06802-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LARG/ARHGEF12 antigen affinity purified polyclonal antibody (Catalog # A06802-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The si2093gnal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LARG/ARHGEF12 at approximately 220 kDa. The expected band size for LARG/ARHGEF12 is at 173 kDa.
Product group Antibodies
Boster Bio
Anti-LARG/ARHGEF12 Antibody Picoband(r)A06802-2-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
TargetARHGEF12
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ARHGEF12 AntibodyLS-C406524
ApplicationsELISA, ImmunoHistoChemistry
TargetARHGEF12
  • SizePrice