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LARS Antibody, HRP conjugated

CSB-PA873736LB01HU
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityHuman
TargetLARS1
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Overview

  • Supplier
    Cusabio
  • Product Name
    LARS Antibody, HRP conjugated
  • Delivery Days Customer
    20
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    HRP
  • Gene ID51520
  • Target name
    LARS1
  • Target description
    leucyl-tRNA synthetase 1
  • Target synonyms
    HSPC192, ILFS1, LARS, LEURS, LEUS, LFIS, LRS, PIG44, RNTLS, cLRS, hr025Cl, leucine--tRNA ligase, cytoplasmic, cytoplasmic leucyl-tRNA synthetase, cytosolic leucyl-tRNA synthetase, leucine tRNA ligase 1, cytoplasmic, leucine translase, proliferation-inducing gene 44
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9P2J5
  • Protein Name
    Leucine--tRNA ligase, cytoplasmic
  • Scientific Description
    Catalyzes the specific attachment of an amino acid to its cognate tRNA in a two step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Exhibits a post-transfer editing activity to hydrolyze mischarged tRNAs.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Western Blot Positive WB detected in: Hela whole cell lysate, Raji whole cell lysate, U251 whole cell lysate All lanes: LARS antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 135, 129, 56 kDa Observed band size: 135 kDa
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Figure 1. Western blot analysis of LARS using anti-LARS antibody (A01109). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LARS antigen affinity purified polyclonal antibody (Catalog # A01109) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LARS at approximately 134KD. The expected band size for LARS is at 134KD.
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